antibody

YB1 (Y-box Binding Protein 1)

Y1500

100 ul

polyclonal

domestic rabbit

nonconjugated

YB1 (Y-box Binding Protein 1)

rabbit

Pab

Antibodies

100 ul

IgG

The Y-box binding protein 1 (YB1) belongs to a family of evolutionarily conserved, multifunctional Y-box proteins that bind single-stranded DNA and RNA and function as regulators of transcription, RNA metabolism, and protein synthesis (1). YB1 binds to Y-box sequences (TAACC) found in multiple gene promoters and can positively or negatively regulate transcription. YB1 activates genes associated with proliferation and cancer, such as cyclin A, cyclin B1, matrix metalloproteinase-2 (MMP-2), and the multi-drug resistance 1 (MDR1) gene (2-4). YB1 represses genes associated with cell death, including the Fas cell death-associated receptor and the p53 tumor suppressor gene (5-7). It also interacts with the RNA- splicing factor SRp30c and stabilizes interleukin 2 mRNA upon induction of T lymphocytes by interleukin 2 (8,9). The majority of YB1 protein localizes to the cytoplasm, with a minor pool found in the nucleus; however, nuclear localization appears to be critical for its role in promoting proliferation. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to the C-terminus of the human YB1 protein. Species Sequence Homology: bovine, xenopus.

Recognizes endogenous levels of total human YB1. Species crossreactivity: mouse, rat, monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloridel, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminus of the human YB1 protein. Species Sequence Homology: bovine, xenopus. Specificity: Recognizes endogenous levels of total human YB1. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IHC WB

-20°C

1. Matsumoto, K. and Wolffe, A.P. (1998) Trends Cell Biol. 8, 318-23, 2. Jurchott, K. et al. (2003) J. Biol. Chem. 278, 27988-96, 3. Mertens, P.R. et al. (1997) J. Biol. Chem. 272, 22905-12, 4. Uchiumi, T. et al. (1993) Cell Growth Differ. 4, 147-57, 5. Lasham, A. et al. (2000) Gene 252, 1-13, 6. Lasham, A. et al. (2003) J. Biol. Chem. 278, 35516-23, 7. Homer, C. et al. (2005) Oncogene 24, 8314-25, 8. Raffetseder, U. et al. (2003) J. Biol. Chem. 278, 18241-8, 9. Chen, C.Y. et al. (2000) Genes Dev. 14, 1236-48, 10. Sutherland, B.W. et al. (2005) Oncogene 24, 4281-92.

https://usbio-images.r.worldssl.net/prodimages/28/Y1500_1.jpg