Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1) | US Biological V2110-18G product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1)

catalog :

V2110-18G

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3i28(7H11)

reactivity :

human

product information

Catalog Number :

V2110-18G

Product wo Prefix :

Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1)

Host :

mouse

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3i28(7H11)

Isotype :

IgG1

Desc1 :

Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing the effects of VEGF into endothelial cells. Upon binding to specific ligands, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of the adaptor proteins, including Shc, Grb-2, PI3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and prouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic peptide corresponding to residues surrounding Tyr951 of human VEGF receptor 2 (KLH coupled).

Specificity :

Detects overexpressed levels of VEGF receptor 2 only when phosphorylated at tyrosine 951. MW: 180kD. Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Mouse

Purity :

Ascites

Form :

Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3i28(7H11) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to residues surrounding Tyr951 of human VEGF receptor 2 (KLH coupled). Specificity: Detects overexpressed levels of VEGF receptor 2 only when phosphorylated at tyrosine 951. MW: 180kD. Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Meyer, M. et al. (1999) EMBO J. 18, 363–374. (2) Dougher-Vermazen, M. et al. (1994) Biochem. Biophys. Res. Commun. 205, 728–738. (3) Kroll, J. and Waltenberger, J. (1997) J. Biol. Chem. 272, 32521–32527. (4) Karkkainen, M.J. and Petrova, T. (2000) Oncogene 19, 5598–5605. (5) Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986–16992.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/19/V2110-18G_1.jpg