antibody

VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2)

V2110-18B

100 ul

polyclonal

domestic rabbit

nonconjugated

VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2)

rabbit

Pab

Antibodies

100 ul

IgG

Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing the effects of VEGF into endothelial cells. Upon binding to specific ligands, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of the adaptor proteins, including Shc, Grb-2, PI3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and prouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4). Applications: Suitable for use in Western Blot. Other applications not teseted. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted V2110-18B in TBS, 5% BSA, 0.1% Tween-20 overnight at 4°C with gentle shaking. Optimal dilutions to be determined by the researcher. Molecular Weight: 180kD Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo

Synthetic phosphopeptide corresponding to residues surrounding Tyr996 of human VEGF receptor 2.

Recognizes overexpressed human VEGFR-2 only when phosphorylated at tyrosine 996. Crossreacts with tyrosine-phosphorylated PDGF receptor, but does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Human and mouse.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr996 of human VEGF receptor 2. Specificity: Recognizes overexpressed human VEGFR-2 only when phosphorylated at tyrosine 996. Crossreacts with tyrosine-phosphorylated PDGF receptor, but does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Human and mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Meyer, M. et al. (1999) EMBO J. 18, 363–374.2. Dougher-Vermazen, M. et al. (1994) Biochem. Biophys. Res. Commun. 205, 728–738.3. Kroll, J. and Waltenberger, J. (1997) J. Biol. Chem. 272, 32521–32527.4. Karkkainen, M.J. and Petrova, T. (2000) Oncogene 19, 5598–5605.5. Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986–16992.

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