antibody

USP15 (Ubiquitin Carboxyl-terminal Hydrolase 15, Ubiquitin Thiolesterase 15, Ubiquitin-specific Processing Protease 15, Deubiquitinating Enzyme 15, Unph-2, Unph4, KIAA0529)

U4100-55E

100 ug

polyclonal

domestic goat

nonconjugated

USP15 (Ubiquitin Carboxyl-terminal Hydrolase 15, Ubiquitin Thiolesterase 15, Ubiquitin-specific Processing Protease 15, Deubiquitinating Enzyme 15, Unph-2, Unph4, KIAA0529)

goat

Pab

Antibodies

100 ug

IgG

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Immunohistochemistry (Paraffin): 2.5-3.8ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Ca Hu Mo Po Rt

Synthetic peptide corresponding to NDIENENCMHTN, from USP15, at C-terminal (NP_006304.1).

Recognizes human USP15. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to NDIENENCMHTN, from USP15, at C-terminal (NP_006304.1). Specificity: Recognizes human USP15. Species sequence homology: Bovine, canine, mouse, porcine and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC

-20°C

1. Baker RT, Wang XW, Woollatt E, White JA, Sutherland GR. Identification, functional characterization, and chromosomal localization of USP15, a novel human ubiquitin-specific protease related to the UNP oncoprotein, and a systematic nomenclature for human ubiquitin-specific proteases. Genomics. 1999 Aug 1;59(3):264-74.

https://usbio-images.r.worldssl.net/prodimages/4/U4100-55E_1.jpg