Ubiquitin C-Terminal Hydrolase L3 (UCH-L3, UCH-30kD) | US Biological U1005-01F product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Ubiquitin C-Terminal Hydrolase L3 (UCH-L3, UCH-30kD)

catalog :

U1005-01F

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

U1005-01F

Product wo Prefix :

Ubiquitin C-Terminal Hydrolase L3 (UCH-L3, UCH-30kD)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Protein ubiquitination and deubiquitination is a reversible process catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies USP, UCH, OTU, MJD and JAMM. UCHL1 and UCHL3 belong to the UCH family of DUBs. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 is more widely distributed (3,4). UCHL1 binds monoubiquitin and UCHL3 shows affinity to both ubiquitin and NEDD8, a ubiquitin-like molecule (5,6). Both enzymes play a role in the regulation of neuronal development and spermatogenesis (4,7,8) and have been implicated in neurodegenerative diseases (3-5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Leu220 of human UCHL3 protein.

Specificity :

Recognizes endogenous levels of human total UCHL3 protein. Species Crossreactivity: mouse, rat, monkey.

Purity :

Purified by peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Leu220 of human UCHL3 protein. Specificity: Recognizes endogenous levels of human total UCHL3 protein. Species Crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Nijman, S.M. et al. (2005) Cell 123:773–86. 2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5:596–613. 3. Leroy, E. et al. (1998) Nature 395:451–2. 4. Kurihara, L.J. et al. (2001) Hum Mol Genet 10:1963–70. 5. Osaka, H. et al. (2003) Hum Mol Genet 12:1945–58. 6. Wada, H. et al. (1998) Biochem Biophys Res Commun 251:688–92. 7. Sakurai, M. et al. (2006) J Cell Sci 119:162–71. 8. Kwon, J. (2007) Exp Anim 56:71–7.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/12/U1005-01F_1.jpg