antibody

TWEAK (TNF-related Weak Inducer of Apoptosis, APO3 Ligand, APO3/DR3 Ligand, APO3L, CD255, DR3LG, FN14, Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12, UNQ181/PRO207)

T9185-01H

100 ul

polyclonal

domestic rabbit

nonconjugated

TWEAK (TNF-related Weak Inducer of Apoptosis, APO3 Ligand, APO3/DR3 Ligand, APO3L, CD255, DR3LG, FN14, Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12, UNQ181/PRO207)

rabbit

Pab

Antibodies

100 ul

IgG

TWEAK (TNFSF12/Apo-3L) is a member of the TNF superfamily of cytokines that are typically involved in immune regulation, inflammation, and apoptosis (1,2). TWEAK mRNA is expressed in a variety of tissues and cell lines, with higher levels observed in the heart, brain, skeletal muscle and within the immune system (1). Like other family members TWEAK is a type II transmembrane protein that can also be proteolytically processed to form a soluble cytokine. Soluble TWEAK is a weak inducer of apoptosis in some cell lines (1). The receptor for TWEAK, known as TWEAKR or fibroblast growth factor inducible 14 (Fn14), is a relatively small member of the TNF receptor family (3). TWEAK signaling has been associated with apoptosis, proliferation, migration, angiogenesis, and inflammation (4). Recent studies have suggested some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity, cancer, and vascular injury (5-8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to residues surrounding His110 of human TWEAK. Species sequence homology: mouse and monkey (100%)

Recognizes recombinant and transfected levels of human TWEAK.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding His110 of human TWEAK. Species sequence homology: mouse and monkey (100%) Specificity: Recognizes recombinant and transfected levels of human TWEAK. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Chicheportiche, Y. et al. (1997) J Biol Chem 272, 32401-10, 2. Marsters, S.A. et al. (1998) Curr Biol 8, 525-8, 3. Wiley, S.R. et al. (2001) Immunity 15, 837-46, 4. Wiley, S.R. and Winkles, J.A. (2005) Cytokine Growth Factor Rev 14, 241-9, 5. Desplat-Jégo, S. et al. (2005) Clin Immunol 117, 15-23, 6. Mueller, A.M. et al. (2005) J Neuroimmunol 159, 55-65, 7. Winkles, J.A. et al. (2006) Cancer Lett 235, 11-7, 8. Ortiz, A. et al. (2009) Cytokine Growth Factor Rev 20, 251-8.

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