Tuberin, phosphorylated (Ser1254) (TSC2, Tuberous sclerosis 2 protein) discontinued | US Biological T9170-06 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Tuberin, phosphorylated (Ser1254) (TSC2, Tuberous sclerosis 2 protein) discontinued

catalog :

T9170-06

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

T9170-06

Product wo Prefix :

Tuberin, phosphorylated (Ser1254) (TSC2, Tuberous sclerosis 2 protein) discontinued

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Tuberin, a product of the tumor suppressor gene TSC2, is important in regulating cell proliferation and tumor development (1). Mutation in either TSC1or TSC2 is responsible for tuberous sclerosis complex (TSC), an autosomal dominant disorder (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB(3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3–5). Additionally, tuberin inhibits the mammalian target of rapamycin (mTOR), resulting in inhibition of p70 S6 kinase and activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and, thus, inhibition of translation (3, 6–7). p38-activated kinase MK2 (MAPKAPK-2) phosphorylates Ser1254 of tuberin, and thus augments the interaction between tuberin and 14-3-3 (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Rt

Immunogen :

Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser1254 of human tuberin.

Specificity :

Detects endogenous levels of tuberin only when phosphorylated at serine 1254. Does not detect tuberin phosphorylated at other sites.

Purity :

Purified by Protein A and immunoaffinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by Protein A and immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser1254 of human tuberin. Specificity: Detects endogenous levels of tuberin only when phosphorylated at serine 1254. Does not detect tuberin phosphorylated at other sites. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653–15658.(2) Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115–119.(3) Manning, B.D. et al. (2002) Mol. Cell 10, 151–161.(4) Aicher, L.D. et al. (2001) J. Biol. Chem. 276, 21017–21021.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/43/T9170-06_1.jpg