Tuberin, phosphorylated (Tyr1571) (TSC2, Tuberous sclerosis 2 protein) (BSA & Azide Free) | US Biological T9170-02A product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Tuberin, phosphorylated (Tyr1571) (TSC2, Tuberous sclerosis 2 protein) (BSA & Azide Free)

catalog :

T9170-02A

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

T9170-02A

Product wo Prefix :

Tuberin, phosphorylated (Tyr1571) (TSC2, Tuberous sclerosis 2 protein) (BSA & Azide Free)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Tuberin, a product of the tumor suppressor gene TSC2, is important in regulating cell proliferation and tumor development (1). Mutation in either TSC1 or TSC2 is responsible for tuberous sclerosis complex (TSC), an autosomal dominant disorder (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). Additionally, tuberin inhibits the mammalian target of rapamycin (mTOR), resulting in inhibition of p70 S6 kinase and activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and, thus, inhibition of translation (3, 6-7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo

Immunogen :

Synthetic phosphopeptide corresponding to residues around Tyr1571 of human tuberin. Species Sequence Homology: human (100%)

Specificity :

Recognizes endogenous levels of human tuberin only when phosphorylated at Tyr1571. Does not detect tuberin phosphorylated at other sites. Species Crossreactivity: mouse and monkey.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2. No preservative and no stabilizing proteins added.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in PBS, pH 7.2. No preservative and no stabilizing proteins added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues around Tyr1571 of human tuberin. Species Sequence Homology: human (100%) Specificity: Recognizes endogenous levels of human tuberin only when phosphorylated at Tyr1571. Does not detect tuberin phosphorylated at other sites. Species Crossreactivity: mouse and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658. 2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119. 3. Manning, B.D. et al. (2002) Mol. Cell 10, 151-161. 4. Aicher, L.D. et al. (2001) J. Biol. Chem. 276, 21017-21021. 5. Dan, H.C. et al. (2002) J. Biol. Chem. 277, 35364-35370. 6. Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967. 7. Inoki, K. et al. (2002) Nat. Cell. Biol. 4, 648-657.