antibody

Tuberin, phosphorylated (Thr1462) (TSC2, Tuberous sclerosis 2 protein)

T9170-01

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Tuberin, phosphorylated (Thr1462) (TSC2, Tuberous sclerosis 2 protein)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors. Tuberin is directly phosphorylated at Thr1462 by Akt/PKB. Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity. In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and eventual inhibition of translation. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation, and that the human TSC complex is a direct biochemical target of the PI3K/Akt pathway. This data complements Drosophila genetics studies suggesting the possible involvement of the tuberin-hamartin complex in the PI3K/Akt mediated insulin pathway. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Ssynthetic phosphopeptide corresponding to residues surrounding Thr1462 of human tuberin. Species Sequence Homology: rat.

Recognizes endogenous levels of human tuberin at ~200kD only when phosphorylated at Thr1462. Does not detect tuberin phosphorylated at other sites. Species Crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Ssynthetic phosphopeptide corresponding to residues surrounding Thr1462 of human tuberin. Species Sequence Homology: rat. Specificity: Recognizes endogenous levels of human tuberin at ~200kD only when phosphorylated at Thr1462. Does not detect tuberin phosphorylated at other sites. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658. 2. Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119. 3. Manning, B.D. et al. (2002) Mol. Cell 10, 151-161. 4. Aicher, L.D. et al. (2001) J. Biol. Chem. 276, 21017-21021. 5. Dan, H.C. et al. (2002) J. Biol. Chem. 277, 35364-35370. 6. Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967. 7. Inoki, K. et al. (2002) Nat. Cell. Biol. 4, 648-657.

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