antibody

Tumor Necrosis Factor Receptor 2 (TNFR2, CD120b)

T9161-21C

100 ul

polyclonal

domestic rabbit

nonconjugated

Tumor Necrosis Factor Receptor 2 (TNFR2, CD120b)

rabbit

Pab

Antibodies

100 ul

IgG

TNF-alpha is an important cytokine produced by numerous cell types including neutrophils, activated lymphocytes, macrophages and NK cells. It plays a critical role in inflammatory responses and in apoptosis (1). TNF-alpha exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-alpha is mediated through two receptors, TNFR1, which is widely expressed, and TNFR2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-alpha have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3). The two receptors for TNF-alpha, TNFR1 (55kD) and TNFR2 (75kD) can mediate distinct cellular responses (4,5). In most cases cytotoxicity elicited by TNF has been reported to act throught TNFR1 (6,7). In contrast, TNFR2 appears to be important in T cell signaling and responses to infection (7,8). TNFR2 binds to distinct members of the TRAF family leading to the activation of NF-kappaB (9,10). Soluble forms of both receptors have also been characterized which can bind TNF-alpha and may play an important role in immune disorders. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide corresponding to residues surrounding Asp339 within the intracellular region of human TNFR2. Species Sequence Homology: monkey, 100%.

Recognizes endogenous levels of total human TNFR2 protein at ~75kD. Species Crossreactivity: mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Asp339 within the intracellular region of human TNFR2. Species Sequence Homology: monkey, 100%. Specificity: Recognizes endogenous levels of total human TNFR2 protein at ~75kD. Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Aggarwal, B.B., Nat. Rev. Immunol. 3: 745-756 (2003). 2. Locksley, R.M., et al., Cell 104: 487-501 (2001). 3. Taylor, P.C., et al., Curr. Opin. Biotech. 15: 557-563 (2004). 4. Tartaglia, L.A., et al., Proc. Natl. Acad. Sci. USA 88: 9292-9296 (1991). 5. Peschon, J.J., et al., J. Immunol. 160: 943-952 (1998). 6. Tartaglia, L.A., et al., Cell 73: 213-216 (1993). 7. Rothe, J., et al., Nature 364: 798-802 (1993). 8. Aspalter, R.M., et al., J. Leukoc. Biol. 74: 572-582 (2003). 9. Rothe, M., et al., Cell 78: 681-692 (1994). 10. Rothe, M., et al., Science 269: 1424-1427 (1995). 11. Humbert, M., et al., Am. J. Respir. Crit. Care Med. 149: 1681-1685 (1994). 12. Schröder, J., et al., Infection 23: 143-148 (1995).

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