Tumor Necrosis Factor alpha (TNFa) | US Biological T9160-04E product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Tumor Necrosis Factor alpha (TNFa)

catalog :

T9160-04E

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H364

reactivity :

human

product information

Catalog Number :

T9160-04E

Product wo Prefix :

Tumor Necrosis Factor alpha (TNFa)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

3H364

Isotype :

IgG1

Desc1 :

Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization. Other applications not tested. Recommended Dilutions: ELISA: 2-8ug/ml as a capture antibody. Western Blot: 1ug/ml detects TNFa in recombinant human TNFa. Immunocytochemistry: 8-25ug/ml detects TNFa in immersion fixed human peripheral blood mononuclear cells (PMBCs) stimulated with LPS and monensin. Neutralization of Bioactivity: 0.04-0.08ug/ml typically neutralizes 50% of the bioactivity of 0.25ng/ml of recombinant human TNFa. Neutralizes TNFa cytotoxicity in L-929 mouse fibroblast cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to aa57-233 (predicted) from human Tumor Necrosis Factor alpha (TNFa) expressed in E. coli (P01375).

Specificity :

Recognizes human Tumor Necrosis Factor alpha (TNFa). Shows <0.05% crossreactivity in sandwich ELISAs with recombinant human TNFb, recombinant mouse TNFa, recombinant rat TNFa and recombinant porcine TNFa.

Purity :

Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/1ug (LAL)

Form :

Supplied as a liquid in PBS, pH 7.2, 5% trehalose.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3H364 Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 5% trehalose. Purity: Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/1ug (LAL) Immunogen: Recombinant protein corresponding to aa57-233 (predicted) from human Tumor Necrosis Factor alpha (TNFa) expressed in E. coli (P01375). Specificity: Recognizes human Tumor Necrosis Factor alpha (TNFa). Shows <0.05% crossreactivity in sandwich ELISAs with recombinant human TNFb, recombinant mouse TNFa, recombinant rat TNFa and recombinant porcine TNFa. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC N WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/44/T9160-04E_1.jpg