antibody

Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) (HRP)

T9155-04C

100 ul

monoclonal

domestic rabbit

HRP

10E54(9F3)

Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) (HRP)

rabbit

Mab

HRP

Antibodies

100 ul

10E54(9F3)

IgG

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Hu Mk Mo Rt Ze

Synthetic peptide corresponding to the N-terminus of human beta-tubulin. Species Sequence Homology: chicken (100%)

Recongnizes endogenous levels of human total Beta-tubulin protein and does not cross-react with recombinant alpha-tubulin. Species crossreactivity: mouse, rat, monkey, bovine, zebrafish.

Purified

Supplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E54(9F3) Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP). Purity: Purified Immunogen: Synthetic peptide corresponding to the N-terminus of human beta-tubulin. Species Sequence Homology: chicken (100%) Specificity: Recongnizes endogenous levels of human total Beta-tubulin protein and does not cross-react with recombinant alpha-tubulin. Species crossreactivity: mouse, rat, monkey, bovine, zebrafish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938-947.

https://usbio-images.r.worldssl.net/prodimages/47/T9155-04C_1.jpg