Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) | US Biological T9154-05K product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

catalog :

T9154-05K

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

T9154-05K

Product wo Prefix :

Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with a/b-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. g-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). Applications: Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (IC): 1:50 Flow Cytometry: 1:25 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Dr Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the sequence of human a-tubulin.

Specificity :

Recognizes endogenous levels of huamn total a-tubulin protein at 52kD., Does not cross-react with recombinant b-tubulin. Species Crossreactivity: mouse, rat, monkey, bovine and Drosophilia.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the sequence of human a-tubulin. Specificity: Recognizes endogenous levels of huamn total a-tubulin protein at 52kD., Does not cross-react with recombinant b-tubulin. Species Crossreactivity: mouse, rat, monkey, bovine and Drosophilia. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938 –947.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/22/T9154-05K_1.jpg

Picture4 Text :

Flow cytometric analysis of C6 cells, using T9154-05K(blue) compared to a nonspecific negative control antibody (red).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/22/T9154-05K_5.jpg

Picture5 Text :

Confocal immunofluorescent analysis of NIH/3T3 cells, using T9154-05K(green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).