antibody

Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

T9154-01C

100 ul

monoclonal

domestic rabbit

nonconjugated

10E52(D20G3)

Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

rabbit

Mab

Antibodies

100 ul

10E52(D20G3)

IgG

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). The Elongator complex catalytic subunit (Elp3) acetylates alpha-tubulin at Lys40 while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-transcriptional modification may be required for dynamic cell shape remod-eling, cell motility, tubulin stability and terminal branching of cortical neurons (2,3). Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:800 Immunoprecipitation: 1:50 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt Ze

A synthetic acetylpeptide corresponding to residues surrounding Lys40 of human alpha-Tubulin. Species sequence homology: Xenopus

Recognizes endogenous levels of human alpha-Tubulin only when acetylated at Lys40, MW: ~52kD. Species Crossreactivity: mouse, rat, monkey and zebrafish.

Purified

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E52(D20G3) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: A synthetic acetylpeptide corresponding to residues surrounding Lys40 of human alpha-Tubulin. Species sequence homology: Xenopus Specificity: Recognizes endogenous levels of human alpha-Tubulin only when acetylated at Lys40, MW: ~52kD. Species Crossreactivity: mouse, rat, monkey and zebrafish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IP WB

-20°C

1. Westermann, S. & Weber, K., Nat. Rev. Mol. Cell Biol. 4: 938-947 (2003). 2. Creppe, C., et al., Cell 136: 551-564 (2009). 3. Hubbert, C., et al., Nature 417: 455-458 (2002).

https://usbio-images.r.worldssl.net/prodimages/18/T9154-01C_1.jpg

Flow Cytometric analysis of HeLa cells, untreated (blue) or treated with Trichostatin A (TSA) (1uM, 16 hours) using T9154-01C. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate) was used as a secondary antibody