Trypsin discontinued | US Biological T8669-13 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Trypsin discontinued

catalog :

T8669-13

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F200

product information

Catalog Number :

T8669-13

Product wo Prefix :

Trypsin discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

6F200

Isotype :

IgG1,k

Desc1 :

Trypsin is a 24kD enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis (2). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:4000 Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbe onto Al(OH)3. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Bo Hu

Immunogen :

Trypsin purified from human pancreatic tissue (1).

Specificity :

Specific for human trypsin, trypsinogen and trypsin in complex with alpha-1-antitrypsin. Crossreacts weakly with bovine trypsin. Epitope differs from that of T8669-13A. Can be used as both catching and detection antibody in sandwich ELISA. Recognizes human trypsin in Western blot under reducing conditions. Has the highest affinity of the trypsin clones available.

Purity :

Purified by Protein A/G affinity chromatography from culture supernatant.

Form :

Supplied as a liquid in PBS, pH 7.4, 0.5M sodium chloride, 15mM sodium azide.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 6F200 Host: mouse Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.5M sodium chloride, 15mM sodium azide. Purity: Purified by Protein A/G affinity chromatography from culture supernatant. Immunogen: Trypsin purified from human pancreatic tissue (1). Specificity: Specific for human trypsin, trypsinogen and trypsin in complex with alpha-1-antitrypsin. Crossreacts weakly with bovine trypsin. Epitope differs from that of T8669-13A. Can be used as both catching and detection antibody in sandwich ELISA. Recognizes human trypsin in Western blot under reducing conditions. Has the highest affinity of the trypsin clones available. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Feinstein G, Hofstein R, Koifmann J, Sokolovsky M Human pancreatic proteolytic enzymes and protein inhibitors. Isolation and molecular properties. Eur J Biochem 43:569-581.(1974) 2. Crossley JR, Smith PA, Edgar BW, Gluckman PD, Elliott RB Neonatal screening for cystic fibrosis, using immunoreactive trypsin assay in dried blood spots. Clin Chim Acta 113:111-121.(1981)