antibody

TrkA, phosphorylated (Tyr490) (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR, Neurotrophin Receptor, NTR)

T8663-04

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

TrkA, phosphorylated (Tyr490) (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR, Neurotrophin Receptor, NTR)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1). Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilution to be determined by the researcher. Molecular weight: 140kD Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr490 of human TrkA.

Recognizes endogenous levels of rat Trk only when phosphorylated at Tyr490. Can also detect the corresponding residues of phosphorylated TrkB and TrkC. Species sequence homology: human, mouse.

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr490 of human TrkA. Molecular weight: 140kD. Specificity: Recognizes endogenous levels of rat Trk only when phosphorylated at Tyr490. Can also detect the corresponding residues of phosphorylated TrkB and TrkC. Species sequence homology: human, mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

1. Segal, R.A. and Greenberg, M.E. (1996) Annu. Rev. Neurosci. 19, 463–489. 2. Stephens, R. M. et al. (1994) Neuron 12, 691–705. 3. Obermeier, A. et al. (1993) EMBO J. 12, 933–941. 4. Obermeier, A. et al. (1994) EMBO J. 13, 1585–1590. 5. Yao, R. and Cooper, G.M. (1995) Science 267, 2003–2006. 6. Binder, D.K. et al. (1999) J. Neurosci. 19, 1424–1436. 7. Gil, C. et al. (2000) FEBS Lett. 481, 177–182. 8. Reuther, G.W. et al. (2000) Mol. Cell. Biol. 20, 8655–8666. 9. Anneren, C. et al. (2000) J. Biol. Chem. 275, 29153–29161. 10. Binder, D.K. et al. (1999) J. Neurosci. 19, 4616–4626.

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