antibody

Transforming Growth Factor beta Induced (BIGH3, TGFBI, Beta-ig-h3)

T8252-91

100 ul

polyclonal

domestic rabbit

nonconjugated

Transforming Growth Factor beta Induced (BIGH3, TGFBI, Beta-ig-h3)

rabbit

Pab

Antibodies

100 ul

IgG

Beta-IG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683aa secretory protein induced by TGF-b that plays a role in cell adhesion, differentiation, and apoptosis (1-4). Beta-IG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxyl terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM Beta-IG-H3 is found in a wide variety of tissues (8-12). Mutations in the Beta-IG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to residues near the C-terminus of human Beta-IG-H3. Species Sequence Homology: monkey (100%)

Recognizes endogenous levels of total human Beta-IG-H3 protein.

Purified by peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues near the C-terminus of human Beta-IG-H3. Species Sequence Homology: monkey (100%) Specificity: Recognizes endogenous levels of total human Beta-IG-H3 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IP WB

-20°C

1. Skonier, J., et al., DNA Cell Biol. 11: 511–522 (1992). 2. Skonier, J., et al., DNA Cell Biol. 13: 571–584 (1994). 3. Hashimoto, K., et al., Biochim. Biophys. Acta. 1355: 303–314 (1997). 4. Kim, J.E., et al., Oncogene 22: 2045–2053 (2003). 5. Kim, J.E., et al., Invest. Ophthalmol. Vis. Sci. 43: 656–661 (2002). 6. Billings, P.C., et al., J. Biol. Chem. 277: 28,003–28,009 (2002). 7. Hanssen, E., et al., J. Biol. Chem. 278: 24,334–24,341 (2003). 8. Gibson, M.A., et al., J. Histochem. Cytochem. 45: 1683–1696 (1997). 9. Billings, P.C., et al., Am. J. Respir. Cell Mol. Biol. 22: 352–359 (2000). 10. Gilbert, R.E., et al., Kidney Int. 54: 1052–1062 (1998). 11. Rawe, I.M., et al., Invest. Ophthalmol. Vis. Sci. 38: 893–900 (1997). 12. LeBaron, R.G., et al., J. Invest. Dermatol. 104: 844–849 (1995). 13. Munier, F.L., et al., Nat. Genet. 15: 247–251 (1997).

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