Transforming Growth Factor beta1 (TGF beta 1, TGFb1, Camurati Engelmann Disease, CED, Diaphyseal Dysplasia 1 Progressive, DPD1, TGFb1, Differentiation inhibiting factor, Cartilage-inducing factor) discontinued | US Biological T8250-16 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Transforming Growth Factor beta1 (TGF beta 1, TGFb1, Camurati Engelmann Disease, CED, Diaphyseal Dysplasia 1 Progressive, DPD1, TGFb1, Differentiation inhibiting factor, Cartilage-inducing factor) discontinued

catalog :

T8250-16

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2Ar2 (9016)

reactivity :

human

product information

Catalog Number :

T8250-16

Product wo Prefix :

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

2Ar2 (9016)

Isotype :

IgG1

Desc1 :

TGF-b1, -2 and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-b1, -2 and -3 are disulfide-linked homodimers. Applications: Suitable for use in Capture ELISA, Neutralization, Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Capture ELISA: 2-8ug/ml. Can be paired with T8250-16B (0.1-0.4ug/ml) as the detection antibody in sandwich ELISA. Neutralization: 0.3-1ug/ml will neutralize 50% of the bioactivity in the presence of 0.25ng/ml of recombinant human TGFb1 and 7.5ng/ml recombinant mouse IL-4. Measured by its ability to neutralize TGF-b1 inhibition of IL-4 dependent proliferation in the HT-2 mouse T cell line. Western Blot: 1ug/ml. Reducing conditions are not recommended. Flow Cytometry (Intracellular): 2.5ug/ml labels 1x10e6 cells. Validated using PC-3 human prostate cancer cell line fixed with paraformaldehyde and permeablized with saponin. Immunocytochemistry: 3-25ug/ml using immersion fixed HEK293 human embryonic kidney cell line Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin embedded sections of human prostate cancer tissue. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Recombinant protein corresponding to human transforming growth factor beta 1 (TGF-b1) and latent TGF-b1 expressed in CHO cells.

Specificity :

Recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide). Neutralizes the biological activity of TGF-b1 and TGF-b1.2. Species Crossreactivity: mouse and rat. In sandwich ELISA, <2% crossreactivity with recombinant human TGF-b3 and recombinant amphibian TGF-b5 was observed and no crossreactivity with recombinant porcine or recombinant human TGFb2 was observed.

Purity :

Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL)

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 2Ar2 (9016) Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS. Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL) Immunogen: Recombinant protein corresponding to human transforming growth factor beta 1 (TGF-b1) and latent TGF-b1 expressed in CHO cells. Specificity: Recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide). Neutralizes the biological activity of TGF-b1 and TGF-b1.2. Species Crossreactivity: mouse and rat. In sandwich ELISA, <2% crossreactivity with recombinant human TGF-b3 and recombinant amphibian TGF-b5 was observed and no crossreactivity with recombinant porcine or recombinant human TGFb2 was observed. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IC IHC N WB

Storage Temperature :

-20°C

Reference :

1. Lin, et al., Cell 68: 775-785 (1992). 2. Tsang, M., et al., Cytokine 7: 389 (1995).