antibody

TRAF Interacting Protein TANK (TRAF Family Member-associated NFkB Activator)

T8171-79B

100 ul

polyclonal

domestic rabbit

nonconjugated

TRAF Interacting Protein TANK (TRAF Family Member-associated NFkB Activator)

rabbit

Pab

Antibodies

100 ul

IgG

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins, forming multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy terminus "TRAF domain" which mediates interactions with associated proteins and many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). At least six TRAFs have been identified (TRAF1-6) and they have been found to act as adaptor proteins for a wide range of cell surface receptors, participating in the regulation of cell survival, proliferation, differentiation and stress responses. TRAF-associated NF- B activator (TANK), also known as TRAF-interacting protein (I-TRAF), is a TRAF binding protein that demonstrates both stimulatory and inhibitory properties (5,6). TANK binds to the carboxy domain of the TRAF1, -2 and -3. Overexpression of TANK prevents the association of TRAF2 with TNFR2, inhibiting TNFR2 and CD40 induced NF- B activation (6). TANK is also reported to synergize with low levels of TRAF2 to activate NF- B (5). TANK assists in the activation of NF- B via association and activation of TANK-binding kinase 1 (TBK1) or IKK which promotes activation of the IKK complex (7,8). It has also been shown that TANK may synergize with TRAF2, TRAF5, and TRAF6 but not TRAF3 in SAPK activation (9). TNF stimulation results in IKK -dependent phosphorylation of TANK which may provide negative feedback regulation of TANK mediated NF- B activation (10). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide corresponding to residues surrounding Ser357 of human TANK. Species sequence homology: monkey, bovine and canine (100%)

Detects endogenous levels of total human TANK protein. It does not crossreact with other TRAF family members. Species Crossreactivity: mouse and rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Ser357 of human TANK. Species sequence homology: monkey, bovine and canine (100%) Specificity: Detects endogenous levels of total human TANK protein. It does not crossreact with other TRAF family members. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

(1) Arch, R.H. et al. (1998) Genes Dev. 12, 2821–2830. (2) Chung, J.Y. et al. (2002) J. Cell Sci. 115, 679–688. (3) Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482–6491. (4) Rothe, M. et al. (1994) Cell 78, 681–692. (5) Cheng, G. and Baltimore, D. (1996) Genes Dev. 10, 963–973. (6) Rothe, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 8241–8846. (7) Pomerantz, J.L. and Baltimore, D. (1999) EMBO J. 18, 6694–6704. (8) Chariot, A. et al. (2002) J. Biol. Chem. 277, 37029–37036. (9) Chin, A.I. et al. (1999) Mol. Cell. Biol. 19, 6665–6672. (10) Bonif, M. et al. (2006) Biochem. J. 394, 593–603.

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