antibody

TLR7 (Toll Like Receptor 7, TLR-7, PRO285, UNQ248) discontinued

T8050-71J

100 ul

polyclonal

domestic rabbit

nonconjugated

TLR7 (Toll Like Receptor 7, TLR-7, PRO285, UNQ248) discontinued

rabbit

Pab

Antibodies

100 ul

IgG

The Toll-like receptors (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1–3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Tollreceptorassociated activator of interferon) and TRAM (Tollreceptor associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which forms a complex with TRAF6, activating TAK1 and IKK. Activation of IKK leads to the degradation of IκB which normally keeps NF-κB inactive by sequestering it in the cytoplasm. TLR7, 8 and 9 form a group of structurally related TLR family members that are are localized to intracellular endosomes (4-6). TLR7 shows highest expression in lung, placenta, and spleen (4). TLR7 mediates responses to a class of synthetic compounds-imidazoquinolines, guanosine-based drugs that induce anti-viral responses (7). Naturally, TLR7 responds to ssRNA viruses to activate NF-κB and trigger IFN production (8-10). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide corresponding to residues near the carboxyl terminus of TLR7.

Detects endogenous levels of total TLR7 protein.

Purified by peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues near the carboxyl terminus of TLR7. Specificity: Detects endogenous levels of total TLR7 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

(1) Akira, S. (2003) J Biol Chem 278, 38105–8. (2) Beutler, B. (2004) Nature 430, 257–63. (3) Dunne, A. and O’Neill, L.A. (2003) Sci STKE 2003, re3. (4) Chuang, T.H. and Ulevitch, R.J. (2000) Eur Cytokine Netw 11, 372–8. (5) Du, X. et al. (2000) Eur Cytokine Netw 11, 362–71. (6) Heil, F. et al. (2003) Eur J Immunol 33, 2987–97. (7) Hemmi, H. et al. (2002) Nat Immunol 3, 196–200. (8) Heil, F. et al. (2004) Science 303, 1526–9. (9) Diebold, S.S. et al. (2004) Science 303, 1529–31. (10) Lund, J.M. et al. (2004) Proc Natl Acad Sci USA 101, 5598-603.

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