Thyroid Peroxidase (TPO) | US Biological T5399-02D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Thyroid Peroxidase (TPO)

catalog :

T5399-02D

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

T5399-02D

Product wo Prefix :

Thyroid Peroxidase (TPO)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Thyroid peroxidase is a key enzyme in thyroid hormone biosynthesis and one of the major antigen in thyroid autoimmune disease. It is a type I glycosylated transmembrane protein localized to the apical part of thyroid follicular cells. It catalyzes iodide oxidation, iodination of tyrosine residues and coupling of iodotyrosines to generate the iodothyronines T4 and T3. The enzymatic reactions of TPO require the presence of iodide, H2O2 and thyroglobulin. The single-copy human gene, located on the short arm of chromosome 2p25 is comprised of 17 exons and 16 introns spanning about 150 kb. Its expression is under the control of thyroid-specific transcription factors such as TTF-1, TTF-2 and PAX. Multiple transcript variants encoding distinct isoforms have been identified for the protein.The pathological role of the protein has been identified in Grave's disease and total iodide organification defect (TIOD). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, observed in human thyroid gland lysates on ~110kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to ELRCGKHQAVGTSPQ, from human TPO, at C-terminal (NP_000538.3, NP_783650.1).

Specificity :

Recognizes human TPO.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to ELRCGKHQAVGTSPQ, from human TPO, at C-terminal (NP_000538.3, NP_783650.1). Specificity: Recognizes human TPO. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/T5399-02D_1.jpg