Thrombin discontinued | US Biological T5045-01C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Thrombin discontinued

catalog :

T5045-01C

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F197

reactivity :

human

product information

Catalog Number :

T5045-01C

Product wo Prefix :

Thrombin discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

6F197

Isotype :

IgG1,k

Desc1 :

Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfide bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin (1). The molecular mass of thrombin is 37kD. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:25, using thrombin directly coated onto plate Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Thrombin isolated from human serum.

Specificity :

Recognizes human thrombin. Also recognizes complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with pro-thrombin. A strong reaction is seen in ELISA with thrombin directly coated onto the microtiterplate. Biotinylation of thrombin blocks antibody binding.

Purity :

Purified by Protein A/G affinity chromatography from culture supernatant.

Form :

Supplied as a liquid in PBS, pH 7.4, 0.5M sodium chloride, 15mM sodium azide.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 6F197 Host: mouse Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.5M sodium chloride, 15mM sodium azide. Purity: Purified by Protein A/G affinity chromatography from culture supernatant. Immunogen: Thrombin isolated from human serum. Specificity: Recognizes human thrombin. Also recognizes complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with pro-thrombin. A strong reaction is seen in ELISA with thrombin directly coated onto the microtiterplate. Biotinylation of thrombin blocks antibody binding. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Fenton JW 2nd, Bing DH (1986) Thrombin active-site regions. Sem Thromb Hemost 12:200-208. 2. Skrzypczak-Jankun E, Carperos V E, Ravichandran K G, Tulinsky A, Westbrook M, Maraganore J M (1991) Structure of the Hirugen and Hirolog 1 complexes of alfa-Thrombin. J.Mol.Biol. 221, 1378-1393 3. Rydel T J, Tulinsky A, Bode W, Huber R (1991) Refined structure of the Hirudin-Thrombin Complex (1991) J. Mol. Biol. 221, 283-601