Thioredoxin 1 | US Biological T5010-10H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Thioredoxin 1

catalog :

T5010-10H

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

7H76(C63C6)

reactivity :

human

product information

Catalog Number :

T5010-10H

Product wo Prefix :

Thioredoxin 1

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H76(C63C6)

Isotype :

IgG

Desc1 :

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase. Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction. A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation. Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1. Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:400. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Ala9 of human Thioredoxin 1.

Specificity :

Recognizes endogenous levels of total human Thioredoxin 1 at ~12kD. Species Crossreactivity: mouse and rat

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H76(C63C6) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Ala9 of human Thioredoxin 1. Specificity: Recognizes endogenous levels of total human Thioredoxin 1 at ~12kD. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC WB

Storage Temperature :

-20°C

Reference :

1. Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14. 2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404. 3. Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606. 4. Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367. 5. Callister, M.E. et al. (2006) Thorax 61, 521-527.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/15/T5010-10H_1.jpg