antibody

TCTP, phosphorylated (Ser46) (Translationally controlled tumor protein, TCTP p23 HRF)

T2040-38

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

TCTP, phosphorylated (Ser46) (Translationally controlled tumor protein, TCTP p23 HRF)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, including a role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by Plk at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF and thus activating the TORC1 pathway, controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction of TCTP expression can cause tumor reversion (12). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100 using citrate as an unmasking buffer. Immunofluorescence (IF-IC): 1:200 Flow Cytometry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein.

Recognizes endogenous levels of human TCTP protein at ~23kD only when phosphorylated at Ser46. In some cell types, may recognizes an 80-100kD protein of unknown origin. Species Crossreactivity: mouse, rat and monkey

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein. Specificity: Recognizes endogenous levels of human TCTP protein at ~23kD only when phosphorylated at Ser46. In some cell types, may recognizes an 80-100kD protein of unknown origin. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IHC WB

-20°C

1. MacDonald, S.M. et al. (1995) Science 269, 688-90, 2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71, 3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65, 4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21, 5. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7, 6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64, 7. Hsu, Y.C. et al. (2007) Nature 445, 785-8, 8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20, 9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print], 10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7, 11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6, 12. Tuynder, M. et al. (2002) Proc Natl Acad Sci U S A 99, 14976-81.

https://usbio-images.r.worldssl.net/prodimages/1/T2040-38_1.jpg

Flow cytometric analysis of Jurkat cells using T2040-38 compared to propidium iodide (DNA content).

https://usbio-images.r.worldssl.net/prodimages/1/T2040-38_5.jpg

Confocal IF analysis of U-2 OS cells using T2040-38 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor is DRAQ5(fluorescent DNA dye).