antibody

TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, 3'-Tab1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, MAP3K7IP1)

T0291-85

100 ul

monoclonal

domestic rabbit

nonconjugated

C25E9

The same clone is also sold as:

• Cell Signaling Technology: 3226

TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, 3'-Tab1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, MAP3K7IP1)

rabbit

Mab

Antibodies

100 ul

C25E9

IgG

TAK1 is a mitogen-activated protein kinase kinase kinase activated by TGF-beta and various pro-inflammatory signals (1,2). In vivo TAK1 activation requires its association with TAK1 binding protein 1 (TAB1), which triggers TAK1 autophosphorylation at Thr184 and Thr187 (3,4). The TAB2 adaptor protein links TAK1 with TRAF6 to mediate TAK1 activation following IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK respectively. TAK1 and TRAF6 also activate the NF-kB pathway by phosphorylating the NF-kB inducing kinase (NIK) to trigger subsequent activation of IKK (2,6). In addition to TAK1, TAB1 interacts with and activates p38alpha (7). Targeted disruption of the TAB1 gene in mice causes a drastic reduction in TAK1 activity and leads to embryonic lethality (8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk

TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1

Synthetic peptide corresponding to residues surrounding Pro381 of human TAB1.

Detects endogenous levels of total human TAB1 protein. Species Crossreactivity: monkey.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: C25E9 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Pro381 of human TAB1. Specificity: Detects endogenous levels of total human TAB1 protein. Species Crossreactivity: monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1 Yamaguchi, K. et al. (1995) Science 270, 2008-2011.2 Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.3 Shibuya, H. et al. (1996) Science 272, 1179-1182.4 Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.5 Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.6 Wang, C. et al. (2001) Nature 412, 346-351.7 Ge, B. et al. (2002) Science 295, 1291-1294.8 Komatsu, Y. et al. (2002) Mech. Dev. 119, 239-249.

https://usbio-images.r.worldssl.net/prodimages/39/T0291-85_1.jpg