antibody

Syk, phosphorylated (Tyr525/526)

S9100-01D

100 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

8A38(C87C1)

Syk, phosphorylated (Tyr525/526)

rabbit

Mab

Phosphorylated

Antibodies

100 ul

8A38(C87C1)

IgG

Syk is a protein tyrosine kinase, that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). Tyrosine 352 of Syk is involved in the association of PLC-g1 (8). Tyrosines 525 and 526 are located in the activation loop of the Syk kinase domain, and phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9). Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:200 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic phospho-peptide corresponding to residues surrounding Tyr525/526 of human Syk. Species Sequence Homology (100%): mouse and rat

Recognizes endogenous levels of Syk protein at ~72kD only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. Detects SyK proein when singly phosphorylated at Tyr526 of human Syk or Tyr520 or Tyr 526 of mouse Syk. Does not crossreact with other tyrosine-phosphorylated protein tyrosine kinases.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 8A38(C87C1) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr525/526 of human Syk. Species Sequence Homology (100%): mouse and rat Specificity: Recognizes endogenous levels of Syk protein at ~72kD only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. Detects SyK proein when singly phosphorylated at Tyr526 of human Syk or Tyr520 or Tyr 526 of mouse Syk. Does not crossreact with other tyrosine-phosphorylated protein tyrosine kinases. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IP WB

-20°C

1. Cheng, A.M. and Chan, A.C. (1997) Curr. Opin. Immunol. 9:528–533. 2. Kurosaki, T. et al. (1997) Curr. Opin. Immunol. 9:309–318. 3. Chu, D.H. et al. (1998) Immunol. Rev. 165:167–180. 4. Turner, M. et al. (2000) Immunol. Today 21:148–154. 5. Coopman, P.J. et al. (2000) Nature 406:742–747. 6. Decker, M. et al. (1998) J. Biol. Chem. 273:8867–8874. 7. Rao, N. et al. (2001) EMBO J. 20:7085–-7095. 8. Law, C.L. et al. (1996) Mol. Cell. Biol. 16:1305–1315. 9. Zhang, J. et al. (2000) J. Biol. Chem. 275:35442–35447.

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