antibody

Stathmin

S7972-50

100 ul

polyclonal

domestic rabbit

nonconjugated

Stathmin

rabbit

Pab

Antibodies

100 ul

IgG

Stathmin, a ubiquitously expressed microtubule destabilizing phosphoprotein, is upregulated in a number of cancers. The N-terminus of the protein is involved in promotion of tubulin filament depolymerization and contains multiple phosphorylation sites. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinase II and IV (1,2) increases upon entrance into the G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase phosphorylation (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3’ and RB3’’. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser38 of human stathmin.

Detects endogenous levels of total human stathmin protein. The antibody does not crossreact with other related proteins such as SCG10, SCLIP and RB3. Species crossreactivity: mouse, monkey and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser38 of human stathmin. Specificity: Detects endogenous levels of total human stathmin protein. The antibody does not crossreact with other related proteins such as SCG10, SCLIP and RB3. Species crossreactivity: mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC WB

-20°C

(1) Marklund, U. et al. (1994) Eur. J. Biochem. 225, 53–60. (2) le Gouvello, S. et al. (1998) J. Immunol. 161, 1113–1133. (3) Mistry, S.J. and Atweh, G.F. (2001) J. Biol. Chem. 276, 31209–31215. (4) Gavet, O. et al. (1998) J. Cell Sci. 111, 3333–3346. (5) Luo, X.N. et al. (1994) J. Biol. Chem. 269, 10312–10318. (6) Vancompernolle, K. et al. (2000) J. Biol. Chem. 275, 33876–33882. (7) Daub, H. et al. (2001) J. Biol. Chem. 276, 1677–1680. (8) Di Paolo, G. et al. (1996) J. Cell. Biol. 133, 1383–1390.

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