STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5) | US Biological S7972-07H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

catalog :

S7972-07H

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

5i114(14H2)

reactivity :

human

product information

Catalog Number :

S7972-07H

Product wo Prefix :

STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

Host :

mouse

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

5i114(14H2)

Isotype :

IgG1

Desc1 :

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation on residue Tyr694 is obligatory for Stat5 activation (1,2). Stat5 has been found to be constitutively active in some leukemic cell types (3). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (4). Stat5alpha and-beta are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-alpha in B cells, but only Stat5alpha is phosphorylated in response to IFN-alpha in HeLa cells (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic phosphopeptide corresponding to residues around tyrosine 694 of mouse Stat5.

Specificity :

Recognizes endogenous levels of mouse Stat5 alpha and Stat5 beta only when phosphorylated at tyrosine 694. It does not crossreact with the corresponding phosphotyrosines of other Stat proteins. Species Crossreactivity: human. Predicted to cross-react with rat based on sequence homology.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 5i114(14H2) Host: mouse Source: mouse Concentration: ~0.2mg/ml Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic phosphopeptide corresponding to residues around tyrosine 694 of mouse Stat5. Specificity: Recognizes endogenous levels of mouse Stat5 alpha and Stat5 beta only when phosphorylated at tyrosine 694. It does not crossreact with the corresponding phosphotyrosines of other Stat proteins. Species Crossreactivity: human. Predicted to cross-react with rat based on sequence homology. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

(1) Gouilleux, F. et al. (1994) EMBO J. 13, 4361–4369. (2) Wakao, H. et al. (1994) EMBO J. 13, 2182–2191. (3) Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855–25861. (4) Dentelli, P. et al. (1999) J. Immunol. 163, 2151–2159. (5) Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937–6944.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/28/S7972-07H_1.jpg