STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5) | US Biological S7972-07F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

catalog :

S7972-07F

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

7H67(C11C5)

reactivity :

human

product information

Catalog Number :

S7972-07F

Product wo Prefix :

STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

Host :

rabbit

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H67(C11C5)

Isotype :

IgG

Desc1 :

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by src upon erythropoietin stimulation (3). Stat5 has been found to be constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5alpha and beta are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-alpha in B cells, but only Stat5alpha is phosphorylated in response to IFN-alpha in HeLa cells (6). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (paraffin): 1:600. Antigen retrieval with citrate buffer is recommended. Flow Cytometry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic peptide corresponding to residues surrounding Tyr694 of Stat5. Species Sequence Homology: Bovine, monkey, rat (100%)

Specificity :

Recognizes endogenous levels of human Stat5 protein when phosphorylated at Tyr694. Species Crossreactivity: mouse

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H67(C11C5) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Tyr694 of Stat5. Species Sequence Homology: Bovine, monkey, rat (100%) Specificity: Recognizes endogenous levels of human Stat5 protein when phosphorylated at Tyr694. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369. 2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191. 3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50. 4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861. 5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159. 6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/10/S7972-07F_1.pdf