antibody

STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)

S7972-07C2

20 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

10E49(D47E7)

STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)

rabbit

Mab

Phosphorylated

Antibodies

20 ul

10E49(D47E7)

IgG

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). Applications: Suitable for use in Western Blot, Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo

Synthetic peptide corresponding to residues surrounding Tyr694 of human Stat5a protein. Species sequence homology: rat and bovine (100%)

Recognizes endogenous levels of human Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. Species Crossreactivity: mouse.

Purified

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E49(D47E7) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Tyr694 of human Stat5a protein. Species sequence homology: rat and bovine (100%) Specificity: Recognizes endogenous levels of human Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. Species Crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF WB

-20°C

1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369, 2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191, 3. Okutani, Y. et al. (2001) Oncogene 20, 6643-6650, 4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861, 5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159, 6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

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