STAT 4 (Signal Transducer and Activator of Transcription 4, STAT4) discontinued | US Biological S7972-02D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

STAT 4 (Signal Transducer and Activator of Transcription 4, STAT4) discontinued

catalog :

S7972-02D

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10E48(2A2)

reactivity :

human

product information

Catalog Number :

S7972-02D

Product wo Prefix :

STAT 4 (Signal Transducer and Activator of Transcription 4, STAT4) discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10E48(2A2)

Isotype :

IgG1

Desc1 :

The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins resulting in their nuclear translocation, binding to specific DNA elements and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to residues 633-652 of human Stat4 protein.

Specificity :

Recognizes human Stat4.

Purity :

Serum

Form :

Supplied in 10 mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 10E48(2A2) Host: mouse Source: human Concentration: Not determined Form: Supplied in 10 mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Purity: Serum Immunogen: Recombinant protein corresponding to residues 633-652 of human Stat4 protein. Specificity: Recognizes human Stat4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Darnell, J.E. et al. (1994) Science 264, 1415-1421, 2. Leonard, W.J. and O’Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322, 3. Zhong, Z. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4806-4810, 4. Yamamoto, K. et al. (1994) Mol. Cell Biol. 14, 4342- 4349, 5. Jacobson, N.G. et al. (1995) J. Exp. Med. 181, 1755- 1762, 6. Bacon, C.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 7307-7311, 7. Thierfelder, W.E. et al. (1996) Nature 382, 171-174, 8. Kaplan, M.H. et al. (1996) Nature 382, 174-177.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/S7972-02D_1.jpg