STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (AlexaFluor®647) | US Biological S7971-01E2 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (AlexaFluor®647)

catalog :

S7971-01E2

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

AF647

antigen modification :

phosphorylated

clone name :

7H65(D3A7)

reactivity :

human

product information

Catalog Number :

S7971-01E2

Product wo Prefix :

STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (AlexaFluor®647)

Host :

rabbit

Product Type :

Mab

Conjugate :

AlexaFluor®647

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H65(D3A7)

Isotype :

IgG

Desc1 :

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Additionally, Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 via the MAPK or mTOR pathway (8,9). Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3alpha (86kD) and Stat3beta (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3beta lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, Add 10ul of the conjugated antibody to 500,000 cells in 90ul PBS/0.5% BSA Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing Alexa Fluor® conjugates will result in a substantial loss of activity. Alexa Fluor® conjugates are sensitive to light.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3. Species sequence homology: hamster, bovine, porcine and equine

Specificity :

Recognizes endogenous levels of mouse Stat3 only when phosphorylated at Tyr705. Does not crossreact with phospho-EGFR or with corresponding phospho-tyrosines of other Stat proteins. Species Crossreactivity: human, monkey and rat

Purity :

Purified prior to conjugation.

Form :

Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H65(D3A7) Host: rabbit Source: mouse Concentration: Not determined Form: Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5. Purity: Purified prior to conjugation. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3. Species sequence homology: hamster, bovine, porcine and equine Specificity: Recognizes endogenous levels of mouse Stat3 only when phosphorylated at Tyr705. Does not crossreact with phospho-EGFR or with corresponding phospho-tyrosines of other Stat proteins. Species Crossreactivity: human, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do Not Freeze

Reference :

Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75-120. Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801-3804. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-115. Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79-85. Bromberg, J.F. et al. (1999) Cell 98, 295-303. Darnell Jr., J.E. et al. (1994) Science 264, 1415-1421. Ihle, J.N. (1995) Nature 377, 591-594. Wen, Z. et al. (1995) Cell 82, 241-250. Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50. Biethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/48/S7971-01E2_1.jpg