antibody

STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

S7971-01E

100 ul

monoclonal

mouse

nonconjugated

phosphorylated

3A27(3E2)

STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

mouse

Mab

Phosphorylated

Antibodies

100 ul

3A27(3E2)

IgG1

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1). It is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4). It possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by tyrosine phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by serine phosphorylation at Ser727, apparently via the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3alpha (86kD) and Stat3beta (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3beta lacks the serine phosphorylation site within the C-terminal transcriptional activation domain (8). Applications: Suitable for use in Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3 (KLH coupled). Species sequence homology: bovine

Recognizes endogenous levels of mouse Stat3 phosphorylated at tyrosine 705. Does not appreciably crossreact with other members of the Stat family. Species Crossreactivity: Human, mouse, rat and monkey

Purified by Protein G and immunoaffinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 3A27(3E2) Host: mouse Source: mouse Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified by Protein G and immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3 (KLH coupled). Species sequence homology: bovine Specificity: Recognizes endogenous levels of mouse Stat3 phosphorylated at tyrosine 705. Does not appreciably crossreact with other members of the Stat family. Species Crossreactivity: Human, mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IP WB

-20°C

1. Heim, M.H., J. Recept. Signal Transduct. Res. 19: 75-120 (1999). 2. Takeda, K., et al., PNAS USA 94: 3801-227 (1997). 3. Catlett–Falcone, R., et al., Immunity 10: 105-115 (1999). 4. Garcia, R., Jove, R., J. Biomed. Sci. 5: 79-85(1998). 5. Bromberg, J.F., et al., Cell 98: 295-303 (1999). 6. Darnell Jr., J.E., et al., Science 264: 1415-1421 (1994). 7. Ihle, J.N., Nature 377: 591-594 (1995). 8. Wen, Z., et al., Cell 82: 241-250 (1995). 9. Yokogami, K., et al., Curr. Biol. 10: 47-50 (2000). 10. Biethahn, S., Exp. Hematol. 27: 885-894 (1999).

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