STAT 1, phosphorylated (Tyr701) (AlexaFluor®488) | US Biological S7969-10A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

STAT 1, phosphorylated (Tyr701) (AlexaFluor®488)

catalog :

S7969-10A

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

AF488

antigen modification :

phosphorylated

clone name :

7H64(58D6)

reactivity :

human

product information

Catalog Number :

S7969-10A

Product wo Prefix :

STAT 1, phosphorylated (Tyr701) (AlexaFluor®488)

Host :

rabbit

Product Type :

Mab

Conjugate :

AlexaFluor®488

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H64(58D6)

Isotype :

IgG

Desc1 :

Stat1, while activated in response to a large number of ligands (1), appears to be essential for responsiveness to IFN-alpha and IFN-gamma (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 has two isoforms, Stat1apha (91kD) and the splice variant Stat1beta (84kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is phosphorylated through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway at Ser727 in response to IFN-alpha and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10, Add 10ul to 500,000 cells in 90ul PBS, 0.5% BSA. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Protect from light. Note: We do not recommend adding glycerol as a cryopreservative.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic phospho peptide corresponding to residues surrounding Tyr701 of human Stat1.

Specificity :

Recognizes endogenous levels of human phospho(Tyr701)Stat1. Also recognizes p91 Phospho(Tyr701)Stat1 and p84 Phospho-Stat1 splice variants. Species Crossreactivity: mouse.

Purity :

Purified prior to conjugation

Form :

Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 488. Fluorophore/Protein ratio: 2-6.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H64(58D6) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 488. Fluorophore/Protein ratio: 2-6. Purity: Purified prior to conjugation Immunogen: Synthetic phospho peptide corresponding to residues surrounding Tyr701 of human Stat1. Specificity: Recognizes endogenous levels of human phospho(Tyr701)Stat1. Also recognizes p91 Phospho(Tyr701)Stat1 and p84 Phospho-Stat1 splice variants. Species Crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do Not Freeze

Reference :

(1) Heim, M.H. (1999) J. Recept. Signal. Transduct. Res. 19, 75–120. (2) Durbin, J.E. et al. (1996) Cell 84, 443–450. (3) Meraz, M.A. et al. (1996) Cell 84, 431–442. (4) Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222–227. (5) Frank, D.A. (1999) Mol. Med. 5, 432–456. (6) Wen, Z. et al. (1995) Cell 82, 241–250.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/47/S7969-10A_1.jpg