STAT 1 (Signal Transducer and Activator of Transcription 1) | US Biological S7969-05B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

STAT 1 (Signal Transducer and Activator of Transcription 1)

catalog :

S7969-05B

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A28(9H2)

reactivity :

human

product information

Catalog Number :

S7969-05B

Product wo Prefix :

STAT 1 (Signal Transducer and Activator of Transcription 1)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3A28(9H2)

Isotype :

IgG1

Desc1 :

Stat1, while activated in response to a large number of ligands (1), appears to be essential for responsiveness to IFN-alpha and IFN-gamma (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 has two isoforms, Stat1alpha (91kD) and the splice variant Stat1beta/Stat2 (84kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors (5). Applications: Suitable for use in ELISA, Western Blot and Immunopreipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide coresponding to STAT1 (KLH). Species sequence homology: bovine.

Specificity :

Recognizes human total Stat1 levels independent of phosphorylation; stronger recognition of non-phosphorylated form. Recognizes Stat1-alpha (91kD) and Stat1-beta (84kD). It does not appreciably crossreact with the other Stat proteins.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3A28(9H2) Host: mouse Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide coresponding to STAT1 (KLH). Species sequence homology: bovine. Specificity: Recognizes human total Stat1 levels independent of phosphorylation; stronger recognition of non-phosphorylated form. Recognizes Stat1-alpha (91kD) and Stat1-beta (84kD). It does not appreciably crossreact with the other Stat proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Heim, M.H. (1999) J. Recept. Signal. Transduct. Res. 19, 75-120. 2. Durbin, J.E. et al. (1996) Cell 84, 443-450. 3. Meraz, M.A. et al. (1996) Cell 84, 431-442. 4. Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227. 5. Frank, D.A. (1999) Mol. Med. 5, 432-456. 6. Wen, Z. et al., (1995) Cell 82: 241-250.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/S7969-05B_1.jpg