STAT 1 (Signal Transducer and Activator of Transcription 1) | US Biological S7969-05A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

STAT 1 (Signal Transducer and Activator of Transcription 1)

catalog :

S7969-05A

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

S7969-05A

Product wo Prefix :

STAT 1 (Signal Transducer and Activator of Transcription 1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Stat1, while activated in response to a large number of ligands (1), appears to be essential for responsiveness to IFN-alpha and IFN-gamma (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 has two isoforms, Stat1alpha (91kD) and the splice variant Stat1beta/Stat2 (84kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors (5). Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide (KLH coupled) derived from the sequence of human Stat1. Species sequence homology: bovine.

Specificity :

Recognizes endogenous levels of total human Stat1 protein. Detects Stat1alpha (91kD) and Stat1beta (84kD). Species Crossreactivity: mouse and rat.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide (KLH coupled) derived from the sequence of human Stat1. Species sequence homology: bovine. Specificity: Recognizes endogenous levels of total human Stat1 protein. Detects Stat1alpha (91kD) and Stat1beta (84kD). Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Heim, M.H. (1999) J. Recept. Signal. Transduct. Res. 19, 75-120. 2. Durbin, J.E. et al. (1996) Cell 84, 443-450. 3. Meraz, M.A. et al. (1996) Cell 84, 431-442. 4. Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227. 5. Frank, D.A. (1999) Mol. Med. 5, 432-456. 6. Peterson, W.M. et al. (2000) J. Neurosci. 20, 4081-4090. 7. Suzuki, K. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 2285-2290. 8. Chen, G. et al. (2001) J. Biol. Chem. 276, 766-772. 9. Hutt, J.A. et al. (2000) J. Biol. Chem. 275, 29123-29131.10. Mahboubi, K. and Pober, J.S. (2002) J. Biol. Chem. 277, 8012-8021.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/S7969-05A_1.jpg