Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src) | US Biological S6504-36B product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src)

catalog :

S6504-36B

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

S6504-36B

Product wo Prefix :

Src, phosphorylated (Tyr527) (pp60Src, pp60c-Src)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The Src family of protein tyrosine kinases is important in the regulation of growth and differentiation of eukaryotic cells (1,2). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of Tyr527 in the C-terminal tail by Csk renders the enzyme less active (3,4). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr527 of human Src.

Specificity :

Recognizes human Src only when phosphorylated at Tyr527. It may crossreact with other Src family members Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites. Species Crossreactivity: Mouse and rat.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr527 of human Src. Specificity: Recognizes human Src only when phosphorylated at Tyr527. It may crossreact with other Src family members Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites. Species Crossreactivity: Mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Brown, M.T. and Cooper, J.A. (1996) Biochim. Biophys. Acta. 1287, 121–149. 2. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell. Dev. Biol. (1997) 13, 513–609. 3. Hunter, T. (1987) Cell 49, 1–4. 4. Nada, S. et al. (1991) Nature 351, 69–72.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/5/S6504-36B_1.jpg