Sox 2 (SOX2, SOX-2, Sex Determining Region Y-box 2, SRY (Sex Determining Region Y) Box 2, SRY-related HMG Box 2, ANOP3, MCOPS3, MGC2413, Transcription Factor SOX2) discontinued | US Biological S5364-11H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Sox 2 (SOX2, SOX-2, Sex Determining Region Y-box 2, SRY (Sex Determining Region Y) Box 2, SRY-related HMG Box 2, ANOP3, MCOPS3, MGC2413, Transcription Factor SOX2) discontinued

catalog :

S5364-11H

quantity :

200 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10E47(D6D9)

reactivity :

human

product information

Catalog Number :

S5364-11H

Product wo Prefix :

Sox 2 (SOX2, SOX-2, Sex Determining Region Y-box 2, SRY (Sex Determining Region Y) Box 2, SRY-related HMG Box 2, ANOP3, MCOPS3, MGC2413, Transcription Factor SOX2) discontinued

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

200 ul

Clone # USB :

10E47(D6D9)

Isotype :

IgG

Desc1 :

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4 and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8). Applications: Suitable for use in ChIP. Other applications not tested. Recommended Dilution: CHIP: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2 protein. Species sequence homology: monkey, bovine, canine and equine (100%)

Specificity :

Recognizes endogenous levels of total human Sox2 protein.

Purity :

Purified

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10E47(D6D9) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2 protein. Species sequence homology: monkey, bovine, canine and equine (100%) Specificity: Recognizes endogenous levels of total human Sox2 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

ChIP

Storage Temperature :

-20°C

Reference :

1. Nichols, J. et al. (1998) Cell 95, 379-391, 2. Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140, 3. Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737, 4. Matin, M.M. et al. (2004) Stem Cells 22, 659-668, 5. Niwa, H. et al. (2000) Nat. Genet. 24, 372-376, 6. Boyer, L.A. et al. (2005) Cell 122, 947-956, 7. Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/21/S5364-11H_1.jpg