antibody

SOCS3 (Suppressor of Cytokine Signaling 3, SOCS-3, Cytokine-inducible SH2 Protein 3, CIS-3, SSI-3, CIS3, SSI3, STAT-induced STAT Inhibitor 3)

S1040-03X

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse, rat

SOCS3 (Suppressor of Cytokine Signaling 3, SOCS-3, Cytokine-inducible SH2 Protein 3, CIS-3, SSI-3, CIS3, SSI3, STAT-induced STAT Inhibitor 3)

goat

Pab

Antibodies

100 ug

IgG

Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family that have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally, the SOCS proteins are composed of an N-terminal region of variable length and aa composition, a central SH2 domain, and a previously unrecognized C-terminal motif that has been called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT- induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 3 is an important regulator of fetal liver hematopoiesis. It is also involved in a broad spectrum of cytokines, e.g. IL-2, IL-3, IL-4, IL-6, Epo, Prolactin, and GH. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 4-6ug/ml, MOLT-4 lysate observed on ~24kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to C-PIREFLDQYDAPL, from human SOCS3, at C-terminal (NP_003946.3). Species sequence homology: Bovine, canine, mouse, porcine and rat.

Recognizes human SOCS3.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

~0.5mg/ml

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-PIREFLDQYDAPL, from human SOCS3, at C-terminal (NP_003946.3). Species sequence homology: Bovine, canine, mouse, porcine and rat. Specificity: Recognizes human SOCS3. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Roberts AW, Robb L, Rakar S, Hartley L, Cluse L, Nicola NA, Metcalf D, Hilton DJ, Alexander WS. Placental defects and embryonic lethality in mice lacking suppressor of cytokine signaling 3. Proc Natl Acad Sci U S A. 2001 Jul 31; 98(16):9324-9.

https://usbio-images.r.worldssl.net/prodimages/29/S1040-03X_1.jpg