antibody

Smad Ubiquitination Regulatory Factor-1 (Smurf1)

S1014-60L

100 ul

polyclonal

domestic rabbit

nonconjugated

Smad Ubiquitination Regulatory Factor-1 (Smurf1)

rabbit

Pab

Antibodies

100 ul

IgG

Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis (1,2). BMP receptors are members of the TGF- beta family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation and activation of these receptors (3-5). Subsequently, they phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the collaborating Smad4 and translocate to the nucleus, where the transcription of target genes is stimulated (5). Smurf1, a member of the HECT family of E3 ubiquitin ligases, seletively interacts with Smads of the BMP pathway, leading to their ubiquitination and degradation (6). In addition, Smurf1 has been shown to interact with the inhibitor Smad, Smad7, the bone-specific transcription factor Runx2/Cbfa1, RhoA and MEKK2 (7-10). In vivo, Smurf1 negatively regulates osteoblast differentiation and bone formation (10,11). A related protein, Smurf2, acts more promiscuously, interacting with both BMP and TGF-b Smad proteins (12). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to a central region within human Smurf1.

Detects endogenous levels of total human Smurf1 protein.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to a central region within human Smurf1. Specificity: Detects endogenous levels of total human Smurf1 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

(1) Hogan, B.L. et al. (1996) Genes Dev. 10, 1580–1594. (2) Hoodless, P.A. et al. (1996) Cell 85, 489–500. (3) Klemm, J.D. et al. (1998) Annu. Rev. Immunol. 16, 569–592. (4) Kretzschmar, M. et al. (1997) Genes Dev. 11, 984–995. (5) Whitman, M. (1998) Genes Dev. 12, 2445–2462. (6) Zhu, H. et al. (1999) Nature 400, 687–693. (7) Ebisawa, T. et al. (2001) J. Biol. Chem. 276, 12477–12480. (8) Zhao, M. et al. (2003) J. Biol. Chem. 278, 27939–27944. (9) Wang, H.R. et al. (2003) Science 302, 1775–1779. (10) Yamashita, M. et al. (2005) Cell 121, 101–113. (11) Zhao, M. et al. (2004) J. Biol. Chem. 279, 12854–12859. (12) Zhang, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 974–979.

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