antibody

Smad4 (MADH4, JIP, DPC4, SMAD4, MAD, Mothers Against Decapentaplegic Homolog 4 (Drosophila), Mothers Against Decapentaplegic, Drosophila, Homolog of, 4, MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 4)

S1014-54D

100 ug

polyclonal

domestic goat

nonconjugated

Smad4 (MADH4, JIP, DPC4, SMAD4, MAD, Mothers Against Decapentaplegic Homolog 4 (Drosophila), Mothers Against Decapentaplegic, Drosophila, Homolog of, 4, MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 4)

goat

Pab

Antibodies

100 ug

IgG

SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-beta, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000 Western Blot: 0.5-2ug/ml, ~60-65kD band seen in mouse NIH-3T3 cell lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See S1014-54D-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Hu Mo Rt

Peptide with sequence C-HTMPIADPQPLD, from the C-terminus of the protein sequence according to NP_005350.1. Species sequence homology: canine, porcine and bovine

Recognizes human SMAD4. Species Crossreactivity: mouse and rat

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

~0.5mg/ml

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Peptide with sequence C-HTMPIADPQPLD, from the C-terminus of the protein sequence according to NP_005350.1. Species sequence homology: canine, porcine and bovine Specificity: Recognizes human SMAD4. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

1. Kim SK, Fan Y, Papadimitrakopoulou V, Clayman G, Hittelman WN, Hong WK, Lotan R, Mao L., DPC4, a candidate tumor suppressor gene, is altered infrequently in head and neck squamous cell carcinoma., Cancer Res 1996 Jun 1; 56(11):2519-21.

https://usbio-images.r.worldssl.net/prodimages/0/S1014-54D_1.jpg