antibody

Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

S1014-53R

100 ul

monoclonal

domestic rabbit

nonconjugated

7H62(C67H9)

Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

rabbit

Mab

Antibodies

100 ul

7H62(C67H9)

IgG

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-beta Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression (9-11). Applications: Suitable for use in ChIP, Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:100 Flow Cytometry: 1:200 Western Blot: 1:1000 Immunoprecipitation: 1:100 ChIP: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues at the N-terminus of Smad3. Species sequence homology: Bovine, xenopus, and zebrafish (100%)

Recognizes endogenous levels of total human Smad3 protein. No cross reactivity is detected with other family members. Species Crossreactivity: mouse, monkey and rat.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide.

Not determined

Product Type: Mab Isotype: IgG Clone No: 7H62(C67H9) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide. Purity: Purified Immunogen: Synthetic peptide corresponding to residues at the N-terminus of Smad3. Species sequence homology: Bovine, xenopus, and zebrafish (100%) Specificity: Recognizes endogenous levels of total human Smad3 protein. No cross reactivity is detected with other family members. Species Crossreactivity: mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

ChIP FC IF IP WB

-20°C

Heldin, C.H. et al. (1997) Nature 390, 465-471. Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194. Derynck, R. et al. (1998) Cell 95, 737-740. Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791. Whitman, M. et al. (1998) Genes Dev. 12, 2445-2462. Wrana, J. (2000) Science 23, 1-9. Attisano, L. and Wrana, J. (2002) Science 296, 1646-1647. Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-4369. Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685. Souchelnytskyi, S. et al. (1997) J. Biol. Chem. 272, 28107-28115. Liu, X. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10669-10674.

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