antibody

SIRT 3 (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)

S1013-88B

100 ul

monoclonal

domestic rabbit

nonconjugated

8A37(C73E3)

SIRT 3 (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)

rabbit

Mab

Antibodies

100 ul

8A37(C73E3)

IgG

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT3, a mammalian homolog of Sir2, exists in human cells in two forms. The full-length 44kD protein localizes to the nucleus, while a processed 28kD protein lacking 142 amino terminal residues localizes exclusively to the mitochondria (2-4). The single murine form of SirT3 is equivalent to the processed human SirT3 protein (2). Full-length SirT3 protein is processed in the mitochondrial matrix by the mitochondrial matrix processing peptidase (MMP) (3). Both full-length and processed forms of SirT3 are enzymatically active and de-acetylate histone H3 at Lys9 and histone H4 at Lys16 in vitro (2). SirT3 also de-acetylates Lys642 of acetyl-CoA synthetase 2 (AceCS2) and activates AceCS2 activity in the mitochondria (5). Restricted caloric intake, which is linked to increased lifespan in multiple organisms, increases SirT3 expression in white and brown adipocytes of obese mice, suggesting a role for SirT3 in aging (6). Two observations implicate SirT3 in the regulation of mitochondrial thermogenesis. First, exposure to cold temperatures increases SirT3 expression in brown adipocytes, while elevated temperatures reduce SirT3 expression (6). Second, over-expression of SirT3 results in increased levels of the mitochondrial uncoupling protein 1 (UCP1) (6). SirT3 protein levels are also elevated in certain breast cancers (7). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Rt

Synthetic peptide corresponding to the sequence of human SIRT 3 protein.

Recognizes endogenous levels of human SIRT 3 protein, both the uncleaved 45kD precursor protein and the cleaved 28kD protein. Does not crossreact with other Sirtuin proteins. Species Crossreactivity: rat and monkey.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 8A37(C73E3) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the sequence of human SIRT 3 protein. Specificity: Recognizes endogenous levels of human SIRT 3 protein, both the uncleaved 45kD precursor protein and the cleaved 28kD protein. Does not crossreact with other Sirtuin proteins. Species Crossreactivity: rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC IP WB

-20°C

1. Guarente, L. (1999) Nat Genet 23, 281–5. 2. Scher, M.B. et al. (2007) Genes Dev 21, 920–8. 3. Schwer, B. et al. (2002) J Cell Biol 158, 647–57. 4. Onyango, P. et al. (2002) Proc Natl Acad Sci USA 99, 13653–8. 5. Schwer, B. et al. (2006) Proc Natl Acad Sci USA 103, 10224–9. 6. Shi, T. et al. (2005) J Biol Chem 280, 13560–7. 7. Ashraf, N. et al. (2006) Br J Cancer 95, 1056–61.

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