SIAH1 (E3 Ubiquitin-protein Ligase SIAH1, Seven in Absentia Homolog 1, Siah-1, Siah-1a, HUMSIAH) | US Biological S1013-42 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

SIAH1 (E3 Ubiquitin-protein Ligase SIAH1, Seven in Absentia Homolog 1, Siah-1, Siah-1a, HUMSIAH)

catalog :

S1013-42

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

product information

Catalog Number :

S1013-42

Product wo Prefix :

SIAH1 (E3 Ubiquitin-protein Ligase SIAH1, Seven in Absentia Homolog 1, Siah-1, Siah-1a, HUMSIAH)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

SIAH1 (seven in absentia homolog 1 (Drosophila), also known as E3 ubiquitin-protein ligase, triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation and a cell surface receptor (DCC) amoungst others. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Ca Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to SRQTATALPTGTSKC, from SIAH1, at N-terminal (NP_003022.3, NP_001006611.1).

Specificity :

Recognizes human SIAH1. Species Crossreactivity: rat. Species sequence homology: Bovine, canine and mouse.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to SRQTATALPTGTSKC, from SIAH1, at N-terminal (NP_003022.3, NP_001006611.1). Specificity: Recognizes human SIAH1. Species Crossreactivity: rat. Species sequence homology: Bovine, canine and mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Sroka K, Voigt A, Deeg S, Reed JC, Schulz JB, Bähr M, Kermer P. BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution. J Neurochem. 2009 Nov;111(3):801-7. 2. Liman J, Sroka K, Dohm CP, Deeg S, Bähr M, Kermer P Modulation of Huntingtin Toxicity by BAG1 is Dependent on an Intact BAG Domain Molecules 2010, 15, 6678-6687;PMID: 0 3. Guigon CJ, Zhao L, Lu C, Willingham MC, Cheng SY. Regulation of beta-catenin by a novel nongenomic action of thyroid hormone beta receptor. Mol Cell Biol. 2008 Jul;28(14):4598-608. 4. Groenman F, Rutter M, Caniggia I, Tibboel D, Post M. Hypoxia-inducible factors in the first trimester human lung. J Histochem Cytochem. 2007 Apr;55(4):355-63. 5. Iwai A, Marusawa H, Matsuzawa S, Fukushima T, Hijikata M, Reed JC, Shimotohno K, Chiba T. Siah-1L, a novel transcript variant belonging to the human Siah family of proteins, regulates beta-catenin activity in a p53-dependent manner. Oncogene. 2004 Sep 30;23(45):7593-600.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/10/S1013-42_1.jpg