antibody

SHP 2, phosphorylated (Tyr580) (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

S1013-29H

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

SHP 2, phosphorylated (Tyr580) (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Tyr580 of human SHP-2. Species Sequence Homology: Chicken.

Recognizes endogenous levels of human SHP 2 at ~72kD only when phosphorylated at Tyr580. Species Crossreactivity: Mouse, rat.

Purified by peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr580 of human SHP-2. Species Sequence Homology: Chicken. Specificity: Recognizes endogenous levels of human SHP 2 at ~72kD only when phosphorylated at Tyr580. Species Crossreactivity: Mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Qu, C.K., Cell Res. 10: 279-288 (2000). 2. Maroun, C.R., et al., Mol. Cell Biol. 20: 8513-8525 (2000). 3. Zhang, S.Q., et al., Mol. Cell Biol. 22: 4062-4072 (2002). 4. Bennett, A.M., et al., Proc. Natl. Acad. Sci. USA 91: 7335-7339 (1994). 5. Lu, W., et al., Mol. Cell 8: 759-769 (2001). 6. Edouard, T., et al., Cell Mol. Life Sci. 64: 1585-1590 (2007).

https://usbio-images.r.worldssl.net/prodimages/0/S1013-29H_1.jpg