antibody

SHP 2, phosphorylated (Tyr542) (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

S1013-29E

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

SHP 2, phosphorylated (Tyr542) (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

SHP2, also known as SHPTP2, Syp, PTP1D and PTP2C, is a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP 2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP 2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP 2 becomes phosphorylated at Tyr542 and Tyr580 in its C-terminus in response to growth factor receptor activation (4). These phosphorylations are proposed to relieve basal inhibition and stimulate its tyrosine phosphatase activity (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Also Available: S1013-29: SHP 2 (SHPTP-2) Pab Rb xMo S1013-29A: SHP 2 Pab Rb xHu S1013-29B: SHP 2 (224-529), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (SHPTP-2, PTPN11) S1013-29E: SHP 2, phosphorylated (Tyr542) Pab Rb xHu S1013-29F: SHP 2, phosphorylated (Tyr580) Pab Rb xHu

Hu Mo Rt

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr542 of human SHP-2. MW: 72kD. Species sequence homology: monkey, chicken, Xenopus.

Detects endogenous levels of human SHP-2 only when phosphorylated at Tyr542. Species Crossreactivity: mouse, rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr542 of human SHP-2. MW: 72kD. Species sequence homology: monkey, chicken, Xenopus. Specificity: Detects endogenous levels of human SHP-2 only when phosphorylated at Tyr542. Species Crossreactivity: mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

(1) Qu, C.K. (2000) Cell Res. 10: 279–288. (2) Maroun, C.R. et al. (2000) Mol. Cell. Biol. 20: 8513–8525. (3) Zhang, S.Q. et al. (2002) Mol. Cell. Biol. 22: 4062–4072. (4) Bennett, A.M. et al. (1994) Proc. Natl. Acad. Sci. USA 91: 7335–7339. (5) Lu, W. et al. (2001) Mol. Cell 8: 759–769.

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