Shh (Sonic HedgeHog) | US Biological S1012-96F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Shh (Sonic HedgeHog)

catalog :

S1012-96F

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

8A36(C9C5)

reactivity :

human

product information

Catalog Number :

S1012-96F

Product wo Prefix :

Shh (Sonic HedgeHog)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

8A36(C9C5)

Isotype :

IgG

Desc1 :

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45kD precursors that undergo auto-cleavage to generate a 19kD amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Rt Ze

Immunogen :

Synthetic peptide corresponding to residues surrounding Glu53 of human Shh. Species Sequence Homology: mouse, Xenopus

Specificity :

Recognizes endogenous levels of total human Shh protein. Does not cross-react with transfected IHH and DHH. Species Crossreactivity: rat and zebrafish

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A36(C9C5) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Glu53 of human Shh. Species Sequence Homology: mouse, Xenopus, zebrafish Specificity: Recognizes endogenous levels of total human Shh protein. Does not cross-react with transfected IHH and DHH. Species Crossreactivity: rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

Benameur, T. et al., (2010) PLoS ONE 5: e12688. doi:10.1371/journal.pone.0012688. 1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087. 2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114. 3. Zhang, X.M. et al. (2001) Cell 106, 781-792. 4. Adolphe, C. et al. (2004) Development 131, 5009-5019. 5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117. 6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/S1012-96F_1.jpg