SFRP4 (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2) | US Biological S1012-89 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

SFRP4 (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2)

catalog :

S1012-89

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

S1012-89

Product wo Prefix :

SFRP4 (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

sFRP4 belongs to the sFRP family whose members contain a cysteine-rich domain homologous to the Wnt-binding domain of the Frizzled proteins. SFRP4 is a circulating protein that can function as a phosphatonin as well as an antagonist of Wnt signaling. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml. Detection limit for rhsFRP-4 is ~2ng/well. Western Blot: 0.1-0.2ug/ml. Detection limit for rhsFRP-4 is ~20ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

NS0-derived rhsFRP-4

Specificity :

Recognizes human sFRP-4. Shows < 5% crossreactivity with rhsFRP-3, rhsFRP-1 and rmsFRP-2.

Purity :

Purified by immunoaffinity chromatography. Endotoxin: < 0.1 EU per 1ug antibody (LAL method).

Form :

Supplied as a liquid in PBS, pH 7.2, 5% trehalose. Also available without trehalose see S1012-89-AF

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 5% trehalose. Also available without trehalose see S1012-89-AF Purity: Purified by immunoaffinity chromatography. Endotoxin: < 0.1 EU per 1ug antibody (LAL method). Immunogen: NS0-derived rhsFRP-4 Specificity: Recognizes human sFRP-4. Shows < 5% crossreactivity with rhsFRP-3, rhsFRP-1 and rmsFRP-2. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811 2. Wolf, V. et al. (1997) FEBS Letters 417:385 3. Drake, J.M. et al. (2003) Apoptosis 8:389 4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452 5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/S1012-89_1.jpg