antibody

SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)

S1012-88

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse

SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)

goat

Pab

Antibodies

100 ug

IgG

Secreted Frizzled Related Protein 3 (SFRP3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, SFRP3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human SFRP3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas. The N-terminal portion of the human protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of SFRP3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of SFRP3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml with the appropriate secondary reagents to detect recombinant human and mouse SFRP3. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo

Recombinant protein corresponding to Ala33-Asn325 of human SFRP3 expressed in NSO cells.

Recognizes human SFRP3. Species Crossreactivity: mouse

Purified by immunoaffinity chromatography. Endotoxin: <0.1EU/ug (LAL)

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

~0.2mg/ml (after reconstitution)

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Endotoxin: <0.1EU/ug (LAL) Immunogen: Recombinant protein corresponding to Ala33-Asn325 of human SFRP3 expressed in NSO cells. Specificity: Recognizes human SFRP3. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Hoang, et al., J. Biol. Chem. 271: 26,131 (1996). 2. Leyns, et al., Cell 88: 747 (1997). 3. Wang, et al., Cell 88: 757 (1997). 4. Mayr, et al., Mech. Dev. 63: 109 (1997). 5. Rattner, et al., PNAS 94: 2859 (1997).