SET7/SET9 | US Biological S1012-27 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

SET7/SET9

catalog :

S1012-27

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

C24B1

The same clone is also sold as:

Cell Signaling Technology: 2825

reactivity :

human

product information

Catalog Number :

S1012-27

Product wo Prefix :

SET7/SET9

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

C24B1

Isotype :

IgG

Desc1 :

SET7/SET9 is a member of the SET domain-containing family, and can specifically methylate Lys4 on histone H3 (1). Like most other lysine-directed histone methyltransferases, it contains a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Methylation of histone H3 Lys4 enhances transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (4,5). In addition, methylation of lysine 4 blocks transcriptional repression by inhibiting the binding of the NURD histone deacetylation complex to the amino-terminal tail of histone H3 and interfering with SUV39H1-mediated methylation of histone H3 Lys9 (1). SET7/SET9 is highly active on free histone H3, but only very weakly methylates H3 within nucleosomes (1). Besides histones, SET7/SET9 also methylates Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (6,7). Methylation of TAF10 stimulates transcription in a promoter-specific manner by increasing the affinity of TAF10 for RNA polymerase II, which may potentiate pre-initiation complex formation (6). Methylation of p53 at Lys372 increases protein stability and leads to upregulation of target genes such as p21. Thus the loss of SET7/SET9 may represent another mechanism for the inactivation of p53 in human cancers (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the N-terminus of the human SET7/SET9 protein.

Specificity :

Recognizes endogenous levels of total human SET7/SET9 protein. Does not cross-react with other SET domain-containing proteins. Species Crossreactivity: mouse, monkey and rat

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: C24B1 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the N-terminus of the human SET7/SET9 protein. Specificity: Recognizes endogenous levels of total human SET7/SET9 protein. Does not cross-react with other SET domain-containing proteins. Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Nishioka, K. et al. (2002) Genes Dev. 16, 479–489. (2) Kubicek, S. et al. (2006) Ernst Schering Res. Found.Workshop , 1–27. (3) Lin, W. and Dent, S.Y. (2006) Curr. Opin. Genet. Dev. 16, 137–142. (4) Wysocka, J. et al. (2006) Nature 442, 86–90. (5) Wysocka, J. et al. (2005) Cell 121, 859–872. (6) Kouskouti, A. et al. (2004) Mol. Cell 14, 175–182. (7) Chuikov, S. et al. (2004) Nature 432, 353–360.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/31/S1012-27_1.jpg